Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence

verfasst von

H. D. Werlein

Abstract

A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm² pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 10⁴ cfu/cm².

Organisationseinheit(en)

Institut für Lebensmittelwissenschaft und Humanernährung

Typ

Artikel

Journal

Meat science

Band

59

Seiten

165-168

Anzahl der Seiten

4

ISSN

0309-1740

Publikationsdatum

10.2001

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Lebensmittelwissenschaften

Elektronische Version(en)

https://doi.org/10.1016/S0309-1740(01)00066-3 (Zugang: Geschlossen)