Comparison of destructively and rinsing gained samples to determine TVC of pig carcasses by bioluminescence
- authored by
- H. D. Werlein
- Abstract
A rinsing-technique with a spraying gun in combination with the ATP-bioluminescence for determining the microbial load was investigated. In each case 3.8-cm2 pig-rind was rinsed with buffered peptone water using a modified sprinkle-bottle. The sample-suspensions were gathered in a one-way syringe and sent through a filter cascade consisting of a coarse filter in order to remove bigger particles and a sensitive filter to attach the micro-organisms as well as somatic cells. This procedure was followed by an extraction of the ATP from the sensitive filter with CellSolver FBC-solution. The destructively gained samples were determined on their TVC by the conventional method. Results of the carcass-surface spray test combined with bioluminescence compared to the conventional destructive method followed by plate-count showed a correlation r=0.93 with a detection limit down to 104 cfu/cm2.
- Organisation(s)
-
Institute of Food Science and Human Nutrition
- Type
- Article
- Journal
- Meat science
- Volume
- 59
- Pages
- 165-168
- No. of pages
- 4
- ISSN
- 0309-1740
- Publication date
- 10.2001
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Food Science
- Electronic version(s)
-
https://doi.org/10.1016/S0309-1740(01)00066-3 (Access:
Closed)
