Optimized high-performance liquid chromatographic procedure for the separation and quantification of the main folacins and some derivatives. I. Chromatographic system

verfasst von

A. Hahn, J. Stein, U. Rump, G. Rehner

Abstract

An improved reversed-phase high-performance liquid chromatographic (HPLC) procedure for the rapid separation and sensitive fluorimetric quantification of the main folacins and some degradation products is presented. The system is the first to include two substances that can be used as internal standards. It allows the complete separation of all substances within 26 min. The fluorescence of reduced folates is measured directly after the column whereas other compounds are converted oxidatively to fluorescent products by post-column derivatization. Among several oxidants tested, potassium peroxodisulphate was found to be the most suitable.

Organisationseinheit(en)

Institut für Lebensmittelwissenschaft und Humanernährung

Externe Organisation(en)

Justus-Liebig-Universität Gießen

Typ

Artikel

Journal

Journal of Chromatography A

Band

540

Seiten

207-215

Anzahl der Seiten

9

ISSN

0021-9673

Publikationsdatum

1991

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Analytische Chemie, Biochemie, Organische Chemie

Elektronische Version(en)

https://doi.org/10.1016/S0021-9673(01)88810-5 (Zugang: Unbekannt)