Optimized high-performance liquid chromatographic procedure for the separation and quantification of the main folacins and some derivatives. I. Chromatographic system

authored by: A. Hahn, J. Stein, U. Rump, G. Rehner
Abstract: An improved reversed-phase high-performance liquid chromatographic (HPLC) procedure for the rapid separation and sensitive fluorimetric quantification of the main folacins and some degradation products is presented. The system is the first to include two substances that can be used as internal standards. It allows the complete separation of all substances within 26 min. The fluorescence of reduced folates is measured directly after the column whereas other compounds are converted oxidatively to fluorescent products by post-column derivatization. Among several oxidants tested, potassium peroxodisulphate was found to be the most suitable.
Organisation(s): Institute of Food Science and Human Nutrition
External Organisation(s): Justus Liebig University Giessen
Type: Article
Journal: Journal of Chromatography A
Volume: 540
Pages: 207-215
No. of pages: 9
ISSN: 0021-9673
Publication date: 1991
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Biochemistry, Organic Chemistry
Electronic version(s): https://doi.org/10.1016/S0021-9673(01)88810-5 (Access: Unknown)