Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

verfasst von

Iriny Ayoub, Marawan A. Elbaset, Mai M. Elghonemy, samir bashandy, Fatma A. A. Ibrahim, Omar Ahmed-Farid, Abdel Nasser Elgendy, Sherif M. Afifi, Tuba Esatbeyoglu, Abdel Razik H. Farrag, mohamed farag, Abdelsamed Elshamy

Abstract

Cyperus species represent a group of cosmopolitan plants used in folk medicine to treat several diseases. In the current study, the phytochemical profile of Cyperus laevigatus ethanolic extract (CLEE) was assessed using UPLC-QTOF–MS/MS. The protective effect of CLEE at 50 and 100 mg /kg body weight (b.w.) was evaluated on hepatorenal injuries induced by thioacetamide (100 mg/kg) via investigation of the extract’s effects on oxidative stress, inflammatory markers and histopathological changes in the liver and kidney. UPLC-QTOF–MS/MS analysis of CLEE resulted in the identification of 94 compounds, including organic and phenolic acids, flavones, aurones, and fatty acids. CLEE improved the antioxidant status in the liver and kidney, as manifested by enhancement of reduced glutathione (GSH) and coenzyme Q10 (CoQ10), in addition to the reduction in malondialdehyde (MDA), nitric oxide (NO), and 8-hydroxy-2′-deoxyguanosine (8OHdG). Moreover, CLEE positively affected oxidative stress parameters in plasma and thwarted the depletion of hepatorenal ATP content by thioacetamide (TAA). Furthermore, treatment of rats with CLEE alleviated the significant increase in plasma liver enzymes, kidney function parameters, and inflammatory markers. The protective effect of CLEE was confirmed by a histopathological study of the liver and kidney. Our results proposed that CLEE may reduce TAA-hepatorenal toxicity via its antioxidant and anti-inflammatory properties suppressing oxidative stress.

Organisationseinheit(en)

Institut für Lebensmittelwissenschaft und Humanernährung
Molekulare Lebensmittelchemie und -entwicklung

Externe Organisation(en)

Ain Shams University
National Research Center, Cairo
National Research Centre (NRC)
National Organization for Drug Control and Research, Cairo
University of Sadat City
Cairo University

Typ

Artikel

Journal

Molecules

Band

27

ISSN

1420-3049

Publikationsdatum

10.2022

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Wirkstoffforschung, Analytische Chemie, Chemie (sonstige), Molekularmedizin, Physikalische und Theoretische Chemie, Pharmazeutische Wissenschaften, Organische Chemie

Elektronische Version(en)

https://doi.org/10.3390/molecules27196470 (Zugang: Offen)