Fractionation and isolation of polyphenols from Aronia melanocarpa by countercurrent and membrane chromatography

verfasst von
T. Esatbeyoglu, M. Rodríguez-Werner, P. Winterhalter
Abstract

In the current study, the fractionation and isolation of polyphenols from Aronia melanocarpa has been performed with two different chromatographic techniques on a large scale. On the one hand, the fractionation of polyphenols such as anthocyanins, phenolic acids, quercetin–glycosides and flavanons from A. melanocarpa pomace was done by high-speed countercurrent chromatography and low-speed rotary countercurrent chromatography. On the other hand, the preseparation of A. melanocarpa extracts from pomace and juice in an anthocyanin and a co-pigment fraction was carried out by membrane chromatography after removing the polymeric procyanidins by precipitation with ethanol. Afterward, the separation and isolation of anthocyanins and co-pigments were done by preparative countercurrent chromatography. Purity control and identification of the isolated compounds were made by HPLC–PDA, HPLC–ESI-MS

n and

1H- as well as

13C-NMR spectroscopy. Various compounds, e.g., chlorogenic acids, isorhamnetin-, apigenin-, luteolin- and taxifolin-derivatives, are described for A. melanocarpa for the first time.

Organisationseinheit(en)
Institut für Lebensmittelwissenschaft und Humanernährung
Molekulare Lebensmittelchemie und -entwicklung
Typ
Artikel
Journal
European Food Research and Technology
Band
243
Seiten
1261-1275
Anzahl der Seiten
15
ISSN
1438-2377
Publikationsdatum
01.07.2017
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ASJC Scopus Sachgebiete
Biotechnologie, Lebensmittelwissenschaften, Allgemeine Chemie, Biochemie, Wirtschaftsingenieurwesen und Fertigungstechnik
Elektronische Version(en)
https://doi.org/10.1007/s00217-016-2837-3 (Zugang: Geschlossen)