Fractionation and isolation of polyphenols from Aronia melanocarpa by countercurrent and membrane chromatography
- verfasst von
- T. Esatbeyoglu, M. Rodríguez-Werner, P. Winterhalter
- Abstract
In the current study, the fractionation and isolation of polyphenols from Aronia melanocarpa has been performed with two different chromatographic techniques on a large scale. On the one hand, the fractionation of polyphenols such as anthocyanins, phenolic acids, quercetin–glycosides and flavanons from A. melanocarpa pomace was done by high-speed countercurrent chromatography and low-speed rotary countercurrent chromatography. On the other hand, the preseparation of A. melanocarpa extracts from pomace and juice in an anthocyanin and a co-pigment fraction was carried out by membrane chromatography after removing the polymeric procyanidins by precipitation with ethanol. Afterward, the separation and isolation of anthocyanins and co-pigments were done by preparative countercurrent chromatography. Purity control and identification of the isolated compounds were made by HPLC–PDA, HPLC–ESI-MS
n and
1H- as well as
13C-NMR spectroscopy. Various compounds, e.g., chlorogenic acids, isorhamnetin-, apigenin-, luteolin- and taxifolin-derivatives, are described for A. melanocarpa for the first time.
- Organisationseinheit(en)
-
Institut für Lebensmittelwissenschaft und Humanernährung
Molekulare Lebensmittelchemie und -entwicklung
- Typ
- Artikel
- Journal
- European Food Research and Technology
- Band
- 243
- Seiten
- 1261-1275
- Anzahl der Seiten
- 15
- ISSN
- 1438-2377
- Publikationsdatum
- 01.07.2017
- Publikationsstatus
- Veröffentlicht
- Peer-reviewed
- Ja
- ASJC Scopus Sachgebiete
- Biotechnologie, Lebensmittelwissenschaften, Allgemeine Chemie, Biochemie, Wirtschaftsingenieurwesen und Fertigungstechnik
- Elektronische Version(en)
-
https://doi.org/10.1007/s00217-016-2837-3 (Zugang:
Geschlossen)