Controlling of Mycobacterium by Natural Degradant-Combination Models for Sequestering Mycolic Acids in Karish Cheese

authored by: Gamal Hamad, Marwa A. Saad, Dalia Talat, Sabria Hassan, Ola M. A. K. Shalabi, Abeer M. Salama, Sarah Abou-Alella, Tuba Esatbeyoglu, Taha Mehany
Abstract: Degradation of the mycobacterial complex containing mycolic acids (MAs) by natural bioactive compounds is essential for producing safe and value-added foods with therapeutic activities. This study aimed to determine the degradation efficiency of natural organic acid extracts (i.e., citric, malic, tartaric, and lactic), quadri-mix extract from fruits and probiotics (i.e., lemon, apple, grape, and cell-free supernatant of Lactobacillus acidophilus), and synthetic pure organic acids (i.e., citric, malic, tartaric, and lactic), against MA in vitro in phosphate buffer solution (PBS) and Karish cheese models. The degradation effect was evaluated both individually and in combinations at different concentrations of degradants (1, 1.5, and 2%) and at various time intervals (0, 6, 12, 24, and 48 h). The results show that MA degradation percentage recorded its highest value at 2% of mixed fruit extract quadri-mix with L. acidophilus and reached 99.2% after 48 h both in PBS and Karish cheese, unlike other treatments (i.e., citric + malic + tartaric + lactic), individual acids, and sole extracts at all concentrations. Conversely, organic acid quadri-mix revealed the greatest MA degradation% of 95.9, 96.8, and 97.3% at 1, 1.5, and 2%, respectively, after 48 h. Citric acid was more effective in MA degradation than other acids. The fruit extract quadri-mix combined with L. acidophilus-fortified Karish cheese showed the highest sensorial characteristics; hence, it can be considered a novel food-grade degradant for MA and could be a promising biocontrol candidate against Mycobacterium tuberculosis (Mtb) in food matrices.
Organisation(s): Institute of Food Science and Human Nutrition
Molecular Food Chemistry and Food Development
External Organisation(s): Arid Lands Cultivation Research Institute
Menofia University, Shibeen El-Kom
Damanhur University, Damanhur
Mansoura University
Port Said University
Alexandria University
Type: Article
Journal: Molecules
Volume: 27
ISSN: 1420-3049
Publication date: 15.12.2022
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Drug Discovery, Analytical Chemistry, Chemistry (miscellaneous), Molecular Medicine, Physical and Theoretical Chemistry, Pharmaceutical Science, Organic Chemistry
Sustainable Development Goals: SDG 3 - Good Health and Well-being
Electronic version(s): https://doi.org/10.3390/molecules27248946 (Access: Open)