A fast and validated method for the determination of malondialdehyde in fish liver using high-performance liquid chromatography with a photodiode array detector

authored by

M. Faizan, T. Esatbeyoglu, B. Bayram, G. Rimbach

Abstract

Malondialdehyde (MDA) is a biomarker of lipid peroxidation and is present in foods and biological samples such as plasma. A high-performance liquid chromatography (HPLC) method was applied to determine MDA in fish liver samples after derivatization with 2,4-dinitrophenylhydrazine (DNPH) using a ODS2 column (10 cm × 4.6 mm, 3 μm) and a photodiode array detector. The mobile phase consisted of 0.2% acetic acid (v/v) in distilled water and acetonitrile (42:58, v/v). The present method was validated in terms of linearity, lower limit of quantification, lower limit of detection, precision, accuracy, recovery, and stability of MDA according to U.S. Food and Drug Administration (FDA) guidelines. The limit of quantification of MDA was 0.39 μmol/L, which is comparable to other methods. The recovery of the spiked MDA liver samples was in the range of 92.4% to 104.2%. This newly modified HPLC method is specific, sensitive, and accurate and allows the analysis of MDA within 4 min in fish liver but also in other tissues and plasma. Practical Application: Malondialdehyde is an established biomarker of lipid peroxidation in foods. We developed a fast, simple, and sensitive HPLC method with photodiode array detection using DNPH for derivatization of malondialdehyde in fish liver.

Organisation(s)

Institute of Food Science and Human Nutrition
Molecular Food Chemistry and Food Development

Type

Article

Journal

Journal of Food Science

Volume

79

Pages

C484-C488

ISSN

0022-1147

Publication date

04.2014

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Food Science

Electronic version(s)

https://doi.org/10.1111/1750-3841.12412 (Access: Closed)