Bioactive Phenolic Compounds from Lingonberry (Vaccinium vitis-idaea L.): Extraction, Chemical Characterization, Fractionation and Cellular Antioxidant Activity

authored by

Tina Kostka, Johanna Josefine Ostberg-Potthoff, Joachim Stärke, Claudia Guigas, Seiichi Matsugo, Valentin Mirčeski, Leon Stojanov, Sanja Kostadinović Veličkovska, Peter Winterhalter, Tuba Esatbeyoglu

Abstract

Lingonberries contain high contents of bioactive compounds such as chlorogenic acids and anthocyanins. In addition to radical scavenging and antioxidant activities, these compounds can protect cells from DNA damage. For this reason, lingonberries might be well suited for nutraceuticals or natural biomedicines. To assess these applications, the present study characterized and identified the most effective extract, only consisting of anthocyanins, copigments or a mixture of both, obtained from a lingonberry juice concentrate. An extract was generated by using a XAD-7 column followed by fractionation into anthocyanins and copigments using adsorptive membrane chromatography. After identification of main polyphenols by HPLC–photodiode array–electrospray ionization–tandem mass spectrometry, free radical scavenging activity was analyzed by electron spin resonance spectroscopy using 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl radicals. Furthermore, cyclic voltammetry analyses and the Trolox equivalent antioxidant capacity (TEAC) assay were applied. Finally, the reactive oxygen species (ROS) reducing effects of the lingonberry extract and its fractions were evaluated in HepG2 cells. While the combination of anthocyanins and copigments possessed the highest antioxidant activities, all samples (XAD-7 extract, anthocyanin and copigment fraction) protected cells from oxidative stress. Thus, synergistic effects between phenolic compounds may be responsible for the high antioxidant potential of lingonberries, enabling their use as nutraceuticals.

Organisation(s)

Molecular Food Chemistry and Food Development
Institute of Food Science and Human Nutrition

External Organisation(s)

Max Rubner-Institut (MRI), Federal Research Institute of Nutrition and Food
Technische Universität Braunschweig
Kanazawa University
University of Lodz
Ss. Cyril and Methodius University
Goce Delcev University (UGD)

Type

Article

Journal

Antioxidants

Volume

11

Publication date

26.02.2022

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Molecular Biology, Biochemistry, Physiology, Clinical Biochemistry, Cell Biology

Sustainable Development Goals

SDG 3 - Good Health and Well-being

Electronic version(s)

https://doi.org/10.3390/antiox11030467 (Access: Open)