Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
- authored by
- Simone Schmidt, Frank Stahl, Kai Oliver Mutz, Thomas Scheper, Andreas Hahn, Jan Philipp Schuchardt
- Abstract
Background: The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3 PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved in oxidative processes. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR). Results: Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases, in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be observed by qRT-PCR analysis. Conclusions: Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3 PUFAs. Further studies combining genetic and metabolic endpoints are needed to verify the regulative effects of n-3 PUFAs in antioxidative gene expression to better understand their beneficial effects in health and disease prevention.
- Organisation(s)
-
Institute of Technical Chemistry
Institute of Food Science and Human Nutrition
Nutrition Physiology and Human Nutrition Section
- Type
- Article
- Journal
- Nutrition and Metabolism
- Volume
- 9
- Pages
- 45
- ISSN
- 1743-7075
- Publication date
- 23.05.2012
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Medicine (miscellaneous), Endocrinology, Diabetes and Metabolism, Nutrition and Dietetics
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Electronic version(s)
-
https://doi.org/10.1186/1743-7075-9-45 (Access:
Open)
https://doi.org/10.1186/1743-7075-9-45 (Access: Unknown)